Discovery of Rare Mutations in Populations: TILLING by Sequencing
نویسندگان
چکیده
منابع مشابه
Discovery of rare mutations in populations: TILLING by sequencing.
Discovery of rare mutations in populations requires methods, such as TILLING (for Targeting Induced Local Lesions in Genomes), for processing and analyzing many individuals in parallel. Previous TILLING protocols employed enzymatic or physical discrimination of heteroduplexed from homoduplexed target DNA. Using mutant populations of rice (Oryza sativa) and wheat (Triticum durum), we developed a...
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Department of Plant Biology and Genome Center (H.T., T.H., B.W., K.J.N., M.L., R.K.T., A.A.K., L.C.), Department of Plant Sciences (R.N., C.U., T.H.T., J.D.), Department of Computer Sciences (V.M., V.F.), Bioinformatics Core, Genome Center (J.F.), and United States Department of Agriculture Agricultural Research Service, Crops Pathology and Genetics Research Unit (T.H.T.), University of Califor...
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TILLING is a method to find mutations in a gene of interest by scanning amplicons from a mutagenized population for sequence changes, commonly a single nucleotide. In the past 5 years, mutation detection by sequencing has become increasingly popular. This chapter details the experimental flow for TILLING-by-Sequencing, highlighting the critical steps involved in tridimensional pooling of genomi...
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High-throughput sequencing of T cell (TCR) receptor repertoires is a promising approach that can be used to characterize the state and dynamics of adaptive immunity and, potentially, to deduce the antigen specificity of the immune response [1]. While the number of applications of TCR profiling is constantly growing, basic protocols have several drawbacks that are making it difficult to utilize ...
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Next-generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of ~1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely proble...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2011
ISSN: 1532-2548
DOI: 10.1104/pp.110.169748